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KMID : 0368419980410010068
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Journal of Plant Biology
1998 Volume.41 No. 1 p.68 ~ p.71
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Cloning and DNA Sequencing of Cytosolic Cu/Zn Superoxide Dismutase Gene from Chinese Cabbage
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Yoon, Keumna
Kim, Hyunuk/Lee, Woosung
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Abstract
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A cDNA clone for the cytosolic Cu/Zn superoxide dismutase (Cu/Zn SOD) from Chinese cabbage (Brassica campestris ssp. pekinensis) was isolated and its DNA sequence was determined. The cDNA clone contains a complete coding sequence which encodes a protein of 152 amino acids and a 3´-untranslated region including a poly A signal. The deduced amino acid sequence shows that it is highly homologous to the Cu/Zn SODs from other plants (60∼90%). The lack of a putative chloroplast targeting transit peptide indicates that the clone represents a cytosolic form of Cu/Zn SOD. Genomic Southern hybridization suggests that cytosolic Cu/Zn SOD genes are present in 1 or 2 copies per genome.
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